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KMID : 1140120100150030248
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Cancer Prevention Research
2010 Volume.15 No. 3 p.248 ~ p.256
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Apoptosis Induction of U937 Human Leukemia Cells by the Ethanol Extracts of Fermented Agabeans
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Lee Hye-Hyeon
Han Min-Ho
Kim Min-Jeong
Seo Min-Jeong
Jeong Yong-Kee
Choi Yung-Hyun
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Abstract
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We previously reported that the extracts of fermented beans exhibits strong anti-inflammatory properties
by suppressing the transcription of pro-inflammatory cytokine genes through the NF-κB signaling pathway however, the mechanisms of their cytoxicities in human cancer cells are poorly understood. In this study, we investigated further possible mechanisms by which fermented beans including soybean (FS), black agabean (FBA) and yellow agabean (FYA) exert their anti-cancer actions in cultured U937 human leukemia cells. Treatment with FS, FBA and FYA in U937 cells resulted in inhibition of growth and induction of apoptosis in a concentration-dependent manner, as measured by MTT assay, fluorescence microscopy, agrose gel electrophoresis and flow cytometry analysis. The increase in apoptosis was associated with up-regulation of pro-apoptotic Bax and Bad expression, down-regulation of anti-apoptotic
Bcl-2 and Bcl-xL expression, and activation of caspases (-3, -8 and -9) and degradation of poly (ADP-ribose) polymerase protein. Furthermore, exposure of FS, FBA and FYA induced the expression of Fas, Fas ligand (FasL), Fas-associated protein with death domain (FADD) and TNFRSF1A-associated via death domain (TRADD). Taken together, our data indicate that the extracts of fermented beans exhibit anti-cancer properties by induction of apoptosis through a signaling cascade of death receptor-mediated extrinsic and mitochondria-mediated intrinsic caspase pathways.
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KEYWORD
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Fermented beans, Apoptosis, Bcl-2, Caspase
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